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Ocular irritation assessed in vitro by a hemolysis and hemoglobin denaturation test

Abstract:

The growing tendency to reduce, refine and replace the experiments with animals, together with the current technological advances, has favored the development of Toxicology in vitro throughout the entire world. As an alternative to the Draize test, an in vitro assay was used to evaluate the potential of ocular irritation by means of hemolysis and the hemoglobin denaturation caused when the red blood cells of known density are treated with the substance at different concentrations. The irritation potentials of twenty cleaning products made by Cuban companies were evaluated. Three of them were classified as moderately irritating, sixteen as irritating and one as very irritating. Three reference substances were used as controls. There was total correspondence with the results of the studies in vivo for evaluated products and substances evaluated. The test showed sensitivity, specificity and good pbkp_redictive capacity when classifying the group of the cleaners as irritants, coinciding with the classification they were given in in vivo assays. The results in vivolin vitro obtained for the reference substances included in the study coincided also. The use of the hemolysis and hemoglobin denaturation test is recommended for a battery of assays intended for toxicity testing of cleaning products that contain surfactants in their formulation.