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Ocular irritation of chemicals measured by three different protocols based on the hen's egg test on the chorioallantoic membrane

Abstract:

Several alternatives have been developed during the past few years for the purpose of replacing the rabbit eye irritation test. The objective of this work was to compare the performance of three different protocols of the hen's egg test on the chorioallantoic membrane (described in an established data bank of in vitro techniques in toxicology), evaluating the irritation potentials of ten chemicals and end products. The data was compared with those obtained in the traditional animal test. There was a good correlation with in vivo data for two of the protocols used, in which three chemicals were classified as non-irritant and seven were considered irritant to a certain degree. However, six false negatives were found with the third test used. Possible causes for these findings are discussed. Among the three assays studied, the best is the one based on the use of trypan blue stain because it is a quicker technique, easier to carry out, and gives a reliable result when evaluating the damages caused in the chorioallantoic membrane in an objective and quantifiable way.