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Optimization in the acclimatization of banana micro propagated seedlings (Musa sp.) using three organic inputs

Abstract:

The successful micropropagation process of banana in vitro plants differs on the acclimatization stage, so there are survival problems at this stage, and it involves several weeks of adaptation. Therefore, the use of efficient microorganisms is a biotechnological alternative for the adaptation of in vitro plants. The present work aims to evaluate the effect of Trichoderma ghanense, arbuscular mycorrhizae, and a liquid biofertilizer in meristematic plants of banana variety William in the acclimatization and vivarium phases. Factorial design 2(k) with 20 treatments were used under greenhouse conditions for six weeks in two phases. The best treatment on the acclimatization phase was B10 increasing the height by 29.6 % and the diameter by 19.9 %, while in the leaf area the best treatment was B19 with 84.7 % increase. Identically, into the nursery phase, the best treatment was B19, with increases of 14.5 % height, 19.3 % diameter of pseudostem, 13.4 % foliar area, 91.8 % root length, 39.98 % wet weight, and 90.5 % dry weight. Finally, in both phases, the mycorrhization percentage was more significant than 45 %. The survival percentages were 100 % during the acclimatization phase. Therefore, it is concluded that bioproducts constitute an alternative for the management and adaptation of plants produced by in vitro methodology.