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Optimization of detection of sexual genotype through microsatellites in populations of Nile Tilapia (Oreochromis niloticus) from the breeding pools

Abstract:

The culture of Nile tilapia (Oreochromis niloticus) is one of the most important aquaculture activities in Ecuador in specific and the world in general, where the cultivation of males is transcendental. The use of breeding with a sexual genotype YY guarantee the viability of male offspring in captivity, avoiding the use of invasive technologies for the environment, and possible interaction with human health. However the absence of rapid detection methods limits the application of this technology. Therefore, the present study was sought optimizing a method for detecting sexual genotypes using microsatellite markers. Genotyping was performed with UNH104, UNH898 and UNH995 microsatellites; of individuals with a known and sexual confirmed genotype, and their relationship was assessed by a test of independence chi-square, and finally the reliability as a diagnostic test was analyzed. Being the UNH898 marker which gave the highest dependency (p = 2.92 x 10-28); showing two alleles (with a size of 264 and 296 bp) present mainly in males, forming a genotype to individuals YY (264/296) and XY (264/296 / X allele). To differentiate males from females this marker showed sensitivity, specificity, PPV and NPV of 96.97%, 82.35%, 91.43% and 93.33% respectively, while differentiating YY from XY individuals; it was about 100.00%, 85.29%, 85.71% and 100.00% respectively.