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PEPTIDE CONTENT AND AMINO ACID COMPOSITION DETERMINATIONS BY THE EZ: FAAST AMINO ACID ANALYSIS PROCEDURE

Abstract:

Methods Peptide sample hydrolysis, derivatization and analysis: acid hydrolysis in vapor phase, with 6M HCl, was used to hydrolyze the samples. The derivatization was performed by using the EZ: faast amino acid analysis procedure (Phenomenex®). Derivatized samples are quickly analyzed by GC-FID. Composition and quantity of hydrolyzed amino acids where determined quickly and accurately. Samples theoretical concentration were prepared by precise weight and dilution in water (aa free).

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    Fuente:

    googlegoogle

    Tipo de documento:

    Other

    Estado:

    Acceso abierto

    Áreas de conocimiento:

    • Bioquímica
    • Química analítica
    • Biomedicina

    Áreas temáticas:

    • Química analítica
    • Bioquímica
    • Fisiología humana

    Contribuidores:

    Vivian Morera CórdovaVivian Morera Córdova