PURIFICATION AND CHARACTERIZATION OF PAPAIN INHIBITORS FROM AMARANTO (Amaranthus caudatus) AND FRIJOL (Phaseolus vulgaris) SEEDS

Abstract:

Plant protease inhibitors are often low molar mass proteins and are present in plant storage and aerial tissues. Their expression is induced in response to damage by insects and pathogenic microorganisms, and the content of these inhibitors can be high in cereal and legume organs. Under the premise that the combination of low and high resolution protein purification techniques allows increasing the specific activity of protease inhibitors present in plant tissues, the objective of this study was to isolate, purify, and biochemically characterize papain inhibitors from amaranth (Amaranthus caudatus) and bean (Phaseolus vulgaris) seeds. Completely randomized experimental designs were used to select low resolution techniques that significantly increase (p <= 0.05) the purification degree of the treated extracts. In all studies, three replicates were made, and measurements were performed in triplicate. Crude protein …

Año de publicación:

2020

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