Pax2+ Astrocytes In The Goldfish Optic Nerve Head During Continuous Growth And Regeneration

Abstract:

Purpose:: During visual system development the optic nerve head (ONH) is a key region that allows the entrance of the retinal ganglion cell axons from the retina to the optic nerve. The ONH has a specific glial cell population which produces numerous molecules involved in the correct formation of the optic nerve, such as the transcription factor Pax2. The fish visual system is in continuous growth and can regenerate because the peripheral retina provides new retinal cells. Thus the adult fish ONH may retain some characteristics similar to those described during development. Methods:: We used goldfish control and injured with a cryo-lesion of the peripheral retina (CL) and an optic nerve crush (OC). They were maintained from 2 to 210 days post-injury, in accordance with the European normative for animal care. We used double immunofluorescence analyses to label astrocytes (Pax2, GFAP, GS, S100, ZO1 and …

Año de publicación:

2011

Keywords:

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