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Peripheral-type benzodiazepine receptors in human prostate

Abstract:

Peripheral benzodiazepine receptors have been evidenced in human normal prostate and prostatic nodular hyperplasia using [3H]Ro 5-4864 and [3H]PK 11195. Binding of both radioligands was found to be saturable. In normal and pathologic tissues, Scatchard analysis revealed a single population of binding sites. K(D) values of [3H]PK 11195 in normal and hyperplastic prostates were 14.6 ± 5.6 and 12 ± 2.42 nmol/l with B(max) of 12,155 ± 1,328 and 13,924 ± 2,454 fmol/mg protein, respectively. Using [3H]Ro 5-4864, the value of B(max) was similar (13,630 ± 1,878 fmol/mg protein), but its affinity was 15 times lower when compared to [3H]PK 11195. Human prostatic nodular hyperplasia is not associated with a change in the density of peripheral benzodiazepine receptors.