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Physiological considerations acting on triplet oxygen for explicit dosimetry in photodynamic therapy

Abstract:

The aims of this study were to determine the spatial and temporal theoretical distribution of the concentrations of Protoporphyrin IX, 3O2 and doses of 1O2. The type II mechanism and explicit dosimetry in photodynamic therapy were used. Furthermore, the mechanism of respiration and cellular metabolism acting on 3O2 were taken into account. The dermis was considered as an absorbing and a scattering medium. An analytical solution was used for light diffusion in the skin. The photophysical, photochemical and biological effects caused by PDT with the initial irradiances of 20, 60 and 150 mW/cm2 were studied for a time of exposure of 20 min and a maximum depth of 0.5 cm. We found that the initial irradiance triples its value in 0.02 cm and that almost 100% of PpIX is part of the dynamics of reactions in photodynamic therapy. Additionally, with about 40 μM of 3O2 there is a balance between the consumed and supplied oxygen. Finally, we determined that with 60 mW/cm2, the highest dose of 1O2 is obtained.