Plant growth promoting bacteria with potential biocontrol agent of Fusarium oxysporum f. sp. lycopersici, and Moniliophthora roreri.

Abstract:

The objective was to characterize the potential PGPR antagonist in the inhibition of mycelial development, conidia in F. oxysporum and spores in M. roreri. The PCR technique was used for the identification of F. oxysporum and M. roreri. Selecting the bacteria: Acinetobacter calcoaceticus BMR2-12, Klebsiella variicola BO3-4, Enterobacter asburiae BA4-19, Enterobacter asburiae PM3-14, Pseudomonas protegens CHA0, Pseudomonas veronii R4 and Bacillus subtilis ATCC-5540. Antagonistic activities were evaluated:(a) Mycelial inhibition in F. oxysporum and M. roreri;(b) Reduction in production of conidia and spores for 12 and 15 days' post-incubation. The PCR confirmed the 670 bp amplification for F. oxysporum. The 750 bp sequencing defined a degree of similarity of 99% to M. roreri, when compared to the NCBI GenBank. The application of B. subtilis is of greater antagonistic activity to inhibition (mycelial and conidia) with (95 and 90%) to F. oxyporum. The activity of BO3-4 in M. roreri totally inhibits mycelial development and spore production 12 and 15 days postincubation respectively. The selection of these biocontrollers and their application as a consortium will offer an alternative to benefit in the reduction of agrochemicals in the cultivation of tomato and cocoa.

Año de publicación:

2019

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