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Plant regeneration of plantain 'Barraganete' from somatic embryos using a temporary immersion system

Abstract:

Banana and plantain (Musa spp.) are highly-demanded fruits at the international level. In vitro propagation of plantain in solid medium by using apical meristems, as initial explants, is a very slow method that is always accompanied by high phenolization of culture medium, which leads to poor shooting or death of cultured tissue. This paper reports the successful regeneration (84.5%) of 'Barraganete' plantain (AAB) plants from somatic embryos, obtained from embryogenic cell suspensions and using a temporary immersion system. The low percentage (1.55%) of somaclonal variation in plants grown under greenhouse conditions indicates that this method could be used for commercial propagation. However, regenerated plants should be monitored for a longer period to rule out the possibility of somaclonal variation in adult plants.