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Preliminary study on the behavior of Aedes albopictus from the central region of Venezuela to chemical insecticides

Abstract:

Aedes albopictus is a competent vector of chikungunya virus, zika and most of the equine virus, capable of transmitting at least 24 arboviruses, susceptible to infection (vertically and horizontally) by serotypes of the dengue virus encephalitis. The objective was to conduct a preliminary study on the behavior against Ae. albopictus organosintetic insecticides from different locations in three central states of the country during the period 2012-2014. Bioassays with insecticide-treated bottles were performed according to the method of the CDC and also biochemical evaluation to identify the mechanisms of detoxification enzyme in vitro and in vivo mechanisms of resistance to the synergist piperonyl butoxide (PB). All strains tested showed sensitivity to DDT (200 ug / mL) and four of them lambdacyhalothrin (6.25 mg / mL), only one, Santos Michelena (SM) presented values of survival. This was also observed: malathion (100 ug / mL) with a mortality time (30 min) of 42.6% for Carabobo; 46.2% Capital District (DC); Zamora 69.6% and 75.5% Mario Briceño Iragorry (MBI) was sensitive only SM. As fenitrothion survival values encountered in the mortalities time (30 min) 72.2%; for Carabobo, 30.7% and 45.8% MBI DC; only expressed sensitivity Zamora and SM. In DC strains Carabobo and the synergistic effect was observed FS = 3 and FS = 1.3, respectively.It is presumed that the survival values found for organophosphate insecticides may be associated with increased esterase alpha (α) and beta (β), to a lesser extent insensitive acetylcholinesterase (ACHEI) and oxidases with survival to lambdacialotrine in strain SM , which could be verified in future studies to be carried out on insecticides resistance. The results found in the present work, constitute the basis for its short-term start in Ae. albopictus from Venezuela and the region, in order to contribute to the effectiveness of chemical control measures of this species of medical importance.