Presence of phospholipase C in coated vesicles from bovine brain Dual regulatory effects of GTP‐analogs

Abstract:

Bovine brain coated vesicles display free calcium‐dependent phospholipase C activity. Gpp(NH)p and GTPγS inhibited phospholipase C at nanomolar concentrations. Increasing concentrations of Gpp(NH)p and GTPγS reversed the inhibitory effects and stimulated phospholipase C activity. Preincubation of coated vesicles with pertussis toxin blocked the poorly‐hydrolyzable GTP‐analogs' inhibitory effects on phospholipase C. These data indicate that guanine nucleotides exert a dual regulatory control of phospholipase C in coated vesicles and that the inhibitory pathway is mediated by a pertussis toxin‐sensitive G‐protein.

Año de publicación:

1991

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Fuente:

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