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Prokaryotic expression and identification of 3-1E gene of merozoite surface antigen of Eimeria acervulina

Abstract:

Eimeria acervulina was isolated from chicken at Hebei province, China. The gene of merozoite surface antigen 3-1E was amplified and cloned into pET28a(+) vector and then transformed into Escherichia coli BL21 strain. Results showed that 3-1E fusion protein band of about 22 kDa was identified by SDS-PAGE. Western blot analysis indicated that the recombinant protein specifically reacted with E. acervulina polyclonal antibody. © Springer-Verlag 2011.

Año de publicación:

2011

Keywords:

    Fuente:

    scopusscopus

    Tipo de documento:

    Article

    Estado:

    Acceso restringido

    Áreas de conocimiento:

    • Microbiología

    Áreas temáticas:

    • Microorganismos, hongos y algas

    Contribuidores:

    Xu P.Xu P.
    Deng B.Deng B.
    Lu J.Lu J.
    Said E.R. AmerSaid E.R. Amer
    Qin J.Qin J.
    Zhao Y.Zhao Y.
    Wang J.Wang J.
    Wang C.M.Wang C.M.
    Bao Y.Bao Y.
    Lu Y.Lu Y.
    He H.X.He H.X.