Pseudothombocytopenia in Total Blood with Ethylenediaminetetraacetic Acid

Abstract:

Pseudothrombocytopenia is an in-vitro phenomenon caused by an interference in automated hematological equipment due to a false reading of platelets counts. Pseudothrombocytopenia’s prevalence in hospitalized patients varies between 0.1 to 2%. In outpatient patients some studies report a prevalence as high as 17%. If pseudothrombocytopenia is not identified it can lead to incongruous clinical decisions making. A cross sectional study with 1401 sample was performed to demonstrate these interferences, these samples were tested by a reference laboratory in Quito, Ecuador. Samples were collected in an occupational health evaluation using anticoagulant EDTA tubes and processed by in an automated analyzer Sysmex XN-3000, equipment that actively participate within Quality Assurance programs established by a Quality Management System with a 3, 9% total error, 3.9% imprecision and-3.8% inaccuracy [6 Sigma]. Thrombocytopenia in this study were 0.7%[IC 95% 0.3-1.1%][10/1.401]. Based in qualitative estimation and post incubation counting, the real thrombocytopenia prevalence was 0.29%[IC 95% 0.01-0.6%][4/1.401] and pseudothombocytopenia was 0.4%[IC 95% 0.07-0.7%][6/1401]. Verification protocols must be implemented in clinical laboratory to its prompt and correct identification. More studies are necessary to correctly identify the prevalence of Pseudothrombocytopenia in hospitalized and outpatient population.

Año de publicación:

2020

Keywords:

Fuente:

google