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Reagentless carbon paste phosphate biosensors: preliminary studies

Abstract:

The development of a reagentless phosphate biosensor with potential applications in continuous monitoring of environmental samples is described. The sensor is based on a biorecognition sequence of three enzymes: phosphorylase A, phosphoglucomutase and glucose 6-phosphate dehydrogenase. The incorporation of these enzymes, the substrate glycogen, the cofactor NAD+, and Os(1,10-phenanthroline-5,6-dione)2Cl2 mediator in a carbon paste electrode covered with in situ formed hydrogels was the fundamental base for the development of an amperometric enzyme electrode for the detection of inorganic phosphate. A study of the effects on the response of enzymatic biosensor response were carried out with dialysis membrane-covered glassy carbon electrodes, achieving a maximum current density of 2 μA cm−2, a detection limit of 6 μM of phosphate with an extended linear dynamic range up to 2 mM …