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Regulated expression and function of CD11c/CD18 integrin on human b lymphocytes. Relation between attachment to fibrinogen and triggering of proliferation through CD11c/CD18

Abstract:

CDllc/CD18 (p150,95) is a β2 integrin expressed by myeloid, natural killer and certain lymphoid cells such as some cytotoxic T cell clones and B cell malignancies. We have studied the expression and function of CD11c on resting and activated B lymphocytes. Flow cytometry, immunoprecipitation, and mRNA analyses showed that cell activation with phorbol esters or with a variety of stimuli such as Staphylococcus aureus or anti-/z antibodies in combination with cytokines induced de novo CDllc/CD18 cell surface expression on most B cells while CDllb expression was not affected. Functional analysis of CDllc/CD18 on B cells revealed that it plays a dual role. First, CDllc/CD18 is implicated in B cell proliferation, as demonstrated by the ability of several anti-CDllc monoclonal antibodies to trigger comitogenic signals; and second, the newly expressed CDllc/CD18 mediates B cell binding to fibrinogen. Our data conclusively demonstrate the role of CDllc/CD18 on both B cell activation and adhesion processes. © 1991, Rockefeller University Press., All rights reserved.