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Resistance to the Antimicrobes and Minimal Inhibitory Concentration of BGNNFG Isolated from non Pasteurized Milk (Raw Milk) II

Abstract:

Four hundred sixteen on pasteurized milk samples (row milk) were taken at random from milk pot at 5 different gathering plants. Gathering plants were located around Lake of Maracaibo basin, Zulia state, Venezuela. Samples were analyzed to determine presence of antibiotics by using the Delvotest-P and the assay disc method of Association of Official analytical chemists (AOAC). Positive samples were cultured to determine gram negative bacterial growth of glucose non-fermentative bacillus (GNGNFB). Susceptibility test to 13 anti-microbes was performed and the minimal inhibitory concentration (MIC) of the stock of microbes in front of 25 anti-microbes was determined. It was found that 48 milk samples had important amount of antibiotics. However, from these samples, there were isolated 10 GNGNFB, from them 7 were Pseudomonas sp and 3 were Acinetobacter sp. Pseudomonas sp showed to be resistant to 10 of the 13 antibiotics tested whereas Acinetobacter sp only was resistant to 5. Microbes stock showed a common pattern of resistance to penicillin, novocaine, cloxacylin and nitrofurantoin. Pseudomonas sp had lower MICs values when exposed to mezlocylin, piperacylin, cefoperazone, ceftazidimine and imipenem. The highest MIC90 for Pseudomonas sp were observed with cefotaxime, ceftriaxone, ampicylim, cefoxitin, cefazolin, cefuroxime cephalotin, vancomycin, ampicylim/sulbactam and amoxicillin/ clavulanic acid. On the other hand, Acinetobacter sp only showed high MIC90 against 6 anti-microbes: cefazolin, cefalotin, vancomycin, penicillin G, oxacillin and clidamycin.