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Restriction maps and sequence homologies of two densovirus genomes

Abstract:

The genomes of Junonia coenia densonucleosis virus (JcDNV) and Galleria mellonella densonucleosis virus (GmDNV) were analysed by restriction endonuclease analysis and Southern blot hybridization. A total of 37 and 33 restriction sites were mapped on JcDNV and GmDNV DNA, respectively, BglI, HaeII and BstEII were site-specific for JcDNV DNA, and BglII and ClaI from GmDNV DNA. The two genomes had nearly identical maps for several restriction endonucleases and Southern blot hybridization using a total genomic JcDNV probe indicated extensive DNA sequence homologies spanning the entire length of the two genomes. Symmetrical cleavage sites, mapping at the extremities of both genomes, confirmed the presence of inverted terminal repeats of at least 420 to 440 bases in length.

Año de publicación:

1990

Keywords:

    Fuente:

    scopusscopus

    Tipo de documento:

    Article

    Estado:

    Acceso abierto

    Áreas de conocimiento:

    • Biología molecular

    Áreas temáticas:

    • Fisiología humana

    Contribuidores:

    Bergoin M.Bergoin M.
    Jourdan M.Jourdan M.
    Eric Louis MialheEric Louis Mialhe
    Compagnon B.Compagnon B.
    Jousset F.X.Jousset F.X.
    Veyrunes J.C.Veyrunes J.C.