SPECIFIC COLLAGEN MOTIFS PROMOTES ADHESION AND INDUCE A PRO-INFLAMMATORY RESPONSE IN FIBROBLASTS OF THE TELEOST FISH GILTHEAD SEABREAM

Abstract:

Collagen (COLs) specific sequences to which mammalian cells can attach, either directly or through protein intermediaries, has been identified using Toolkits of triple-helical peptides and GXX’GEX motifs within the COLs. We have previously reported that COL1 was able to prime in vitro the respiratory burst and induce a specific set of immune-and ECM-related molecules in phagocytes of the teleost gilthead seabream (Sparus aurata L.). In the present study, we used the seabream fibroblast cell line SAF-1 to identify the binding COL motifs by end-point and real-time cell adhesion assays against the Toolkits of COL peptides. We identified COL motifs involved in the early adhesion of these cells in a magnesium-dependant way. Furthermore, we found that GFOGER and GLOGEN motifs (where O is hydroxyproline) in suspension were able to induce the expression of interleukin-1beta and cyclooxygenase-2 genes. These data suggest that the early mechanisms of collagen recognition, adhesion and inflammatory response would differ depending on the specific GXX’GEX” motifs.

Año de publicación:

2010

Keywords:

Fuente:

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