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Salmonella prevalence in poultry farms of Ecuador and serotype identification based on multiplex PCR systems

Abstract:

Salmonellosis control is in part based on the reduction of Salmonella presence in primary poultry production. The aim of this study was to determine the prevalence of Salmonella in poultry farms of Ecuador. In 2014, the first national survey of industrialized poultry farms was done. Salmonella spp. was isolated from manure samples and serotypes were determined by conventional serotyping. Additionally, the ability of multiplex Polymerase Chain Reaction (PCR) systems to identify the Salmonella serotype of the isolates was evaluated and results were confirmed with serological methods. One hundred forty five farms were sampled and a total of 26 isolates of Salmonella spp. were recovered from 16 of them, resulting in a national apparent prevalence at the farm level of 11%; 12.7% in broiler chickens farms, 11.5% in breeder flocks and 8.9% in egg-laying hens farms. The isolated serotypes of Salmonella enterica subsp. enterica were: Enteritidis, Infantis, Saintpaul and Serogroup O:8:Z4 Complex. A flow diagram for the use of the evaluated multiplex PCR systems is proposed and the potential significances of the identified serotypes for public health are discussed.