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Search of invariant prot ein in Zea mays under biotic stress caused by Aspergillus flavus

Abstract:

Plant pathogen attacks result in numerous biochemical responses that determine the plant ability to fight the aggression. Maize (Zea mays) crop improvement requires an agronomic management in which the studies of infection by the pathogen at the molecular level are important, having focused on the study of genes and proteins induced by the pathogen. However, the invariant proteins present and their possible role in resistance, as they can determine the maintenance of the plant against infection, have not been characterized. Proteomic tools were used to identify invariant proteins in protein profiles of grains from resistant and susceptible corn lines under biotic stress after being inoculated with the fungus Aspergillus flavus. Selected spots were subjected to in situ digestion with trypsin followed by subsequent identification by MALDI-TOF mass spectrometry. Twenty proteins were selected that were present in all conditions and after identification through databases it was determined that 50% of them may be associated with metabolism (isoprenoid synthesis, mevalonate pathway and cell cycle), 40% with stress conditions (production of reactive oxygen species, water stress and pathogenicity), and 10% with hypothetical proteins of unknown function. These results provide more complete proteome information to facilitate future research.