Selective Enzymatic Oxidations by using Oxygen as oxidizing agent: Immobilization and Stabilization of FNR, a NADP⁺ regenerating enzyme

Abstract:

We have developed a new strategy to prepare very active and very stable derivatives of the enzyme ferredoxin-NADP+-reductase (FNR). This strategy is based on a very controlled process of multipoint covalent attachment between the enzyme, through its amino groups, and very dense monolayers of linear aldehyde groups on support surfaces. Because of the excellent properties of this enzyme and the ones of its derivatives, we have been able to regenerate a complex and interesting enzymatic cofactor, NADP+, by using molecular oxygen as oxidizing agent and under a very wide range of experimental conditions. In this way, by coupling our efficient oxidative system to a number of NADP-depending redox enzymes, we should be able to develop very specific and very selective oxidative processes (dehydrogenation, deaminations, hydroxylations…) under very mild oxidizing conditions (e.g. at room temperature and by using molecular oxygen as unique oxidizing agent). © 1994, Elsevier Science B.V.

Año de publicación:

1994

Keywords:

Fuente:

scopus