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Short report: Molecular mass screening to incriminate sand fly vectors of Andean-type cutaneous leishmaniasis in Ecuador and Peru

Abstract:

Sand flies from the Andean areas of Ecuador and Peru were examined for Leishmania infections by using our recently established molecular mass screening method. Leishmanial minicircle DNA-positive sand flies were detected in 3 of 192 and 1 of 462 samples from Ecuador and Peru, respectively. Sand fly species were identified by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the 18S ribosomal RNA (rRNA) gene, and the positive flies were Lutzomyia (Lu.) ayacuchensis and Lu. peruensis, respectively. Furthermore, cytochrome b and mannose-phosphate isomerase gene sequence analyses identified the parasites from Ecuador and Peru as Leishmania (Leishmania) mexicana and L. (Viannia) peruviana, respectively. Thus, the mass screening method was confirmed to be a powerful tool for sand fly research. Copyright © 2008 by The American Society of Tropical Medicine and Hygiene.

Año de publicación:

2008

Keywords:

    Fuente:

    scopusscopus

    Tipo de documento:

    Article

    Estado:

    Acceso restringido

    Áreas de conocimiento:

    • Parasitología
    • Microbiología

    Áreas temáticas:

    • Microorganismos, hongos y algas
    • Fisiología y materias afines
    • Enfermedades

    Contribuidores:

    Iwata H.Iwata H.
    Jorge Diego MarcoJorge Diego Marco
    Paola Andrea BarrosoPaola Andrea Barroso
    Eduardo A. Gomez-LandiresEduardo A. Gomez-Landires
    Uezato H.Uezato H.
    Caceres A.G.Caceres A.G.
    Mimori T.Mimori T.
    Kato H.Kato H.
    Hashiguchi Y.Hashiguchi Y.