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Similarities between ovarian and bidderian oocytes

Abstract:

In vertebrate embryos, the gonadal primordium is colonized by the primordial germ cells and differentiates into either testis or ovary. Nonetheless, the anterior portion of the gonadal primordium fails to develop into testis in male bufonids resulting in an undeveloped ovary known as Bidder's organ. Bidderian oocytes grow and undergo vitellogenesis if testes are removed. The authors compared oogenesis between Bidder's organ and ovary in Bufo marinus by analyzing morphology and differentiated expression of the lamina-associated polypeptide 2 (LAP2). Isoforms of LAP2 are specific markers of frog germ and somatic cell differentiation. Bidderian oocyte growth was studied in male Bufo marinus for three months. Oocytes from Bidder's organ and ovary were examined with sodium dodecylsulfate polacrylamide gel electrophoresis and immunoblotting. Based on the Xenopus laevis staging of oogenesis, the authors …