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Skin-external device integration using porous cationic poly(DMAA-co-AMTAC) hydrogels

Abstract:

Porous poly(DMAA-co-AMTAC) hydrogels (N,N-Dimethylacrylamide (DMAA) copolymerized with (3-Acrylamidopropyl)-trimethylammonium chloride (AMTAC)), fabricated using the inverted colloid crystal method, were integrated with living human skin. Full thickness human breast skin expiants discarded from surgeries were cultured for five days at the air-liquid interface using a Transwell culture system. Cylindrical, disks or other shaped hydrogels were placed inside the skin expiants fitting the punctures produced by punch biopsies. Full section histological analysis of the skin explants with the inserted hydrogel was then performed. Results indicate that poly(DMAA-co-AMTAC) hydrogels induce substantial extracellular matrix material deposition, maintain excellent dermal integrity in the contact areas with the skin and induce dermal fibers to integrate into the 190 μm hydrogel pores. © 2009 Materials Research Society.