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Spores germination and gametophytes of alsophila odonelliana (cyatheaceae) in diferent sterile media

Abstract:

The tree fern Alsophila odonelliana is found from southern Bolivia to northwestern Argentina, in the Tucuman-Bo-livian forests. Spore germination was tested in three sterile nutritive media: Dyer (D), Murashige and Skoog (MS) and Knudson (K). We also evaluated the efect on gametophyte development of the addition of two growth regulators, BAP and 2,4-D to the culture medium Dyer; 2,4-D was evaluated using two concentrations: 0.1 and 2.0 mg/L, and BAP using three concentrations, 0.01; 0.1 and 1.0 mg/L. We found no signifcant diferences in the germination of spores among all three media. We noted that supplementing media with BAP afected the longitudinal growth of the fla-mentous gametophytes, and the proliferation of ramifcations from early stages. Supplementation with 2,4-D led to the development of short flamentous gametophytes and ameristic laminar forms. Both regulators afected the structure of male gametangia. Tere was not 2,4-D somatic embryogenesis as in other plants.