Stepwise surface tailoring of carbon nanotubes with polyelectrolyte brushes and lipid layers to control their intracellular distribution and “in vitro” toxicity

Abstract:

Carbon Nanotubes (CNTs) have been functionalized with a layer of poly(sulfopropyl methacrylate) (PSPM) synthesized from silane initiators attached to the CNT walls. The PSPM brush was coated with a layer of poly(allyl amine hydrochloride) (PAH) and on top of the PAH layer lipid vesicles composed of 75% 1,2-dioleoyl-sn-glycero-3-phosphocholine and 25% 1,2-dioleoyl-sn-glycero-3-[phospho-L-serine] were assembled. The surface modification of the CNTs and lipid assembly were followed by Transmission Electron Microscopy (TEM) and ζ-potential measurements. TEM and Confocal Raman Microscopy (CRM) were used to study the uptake and localization of the surface modified CNTs in HepG2 cells. PSPM modified CNTs were present in the cytoplasm. Proliferation studies based on the MTT assay were used to assess toxicity of the CNTs for the different modifications. Cell proliferation decreases for oxidized …

Año de publicación:

2011

Keywords:

Fuente:

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