Structural and dynamical aspects of skin studied by multiphoton excitation fluorescence microscopy-based methods This article is dedicated to the loving memory of Prof. Robert (Bob) Clegg

Abstract:

This mini-review reports on applications of particular multiphoton excitation microscopy-based methodologies employed in our laboratory to study skin. These approaches allow in-depth optical sectioning of the tissue, providing spatially resolved information on specific fluorescence probes' parameters. Specifically, by applying these methods, spatially resolved maps of water dipolar relaxation (generalized polarization function using the 6-lauroyl-2-(N,N-dimethylamino)naphthale probe), activity of protons (fluorescence lifetime imaging using a proton sensitive fluorescence probe - 2,7-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein) and diffusion coefficients of distinct fluorescence probes (raster imaging correlation spectroscopy) can be obtained from different regions of the tissue. Comparative studies of different tissue strata, but also between equivalent regions of normal and abnormal excised skin, including applications of fluctuation correlation spectroscopy on transdermal penetration of liposomes are presented and discussed. The data from the different studies reported reveal the intrinsic heterogeneity of skin and also prove these strategies to be powerful noninvasive tools to explore structural and dynamical aspects of the tissue. © 2012 Elsevier B.V. All rights reserved.

Año de publicación:

2013

Keywords:

• LAURDAN generalized polarization function
• Multiphoton excitation fluorescence microscopy
• fluorescence lifetime imaging
• Raster image correlation spectroscopy

Fuente:

scopus