Structure elucidation of NeuAc, NeuGc and Kdn-containing O-glycans released from Triturus alpestris oviductal mucins - Characterization of the poly LacdiNAc sequence: HSO3(4)(GalNAcβ1-4GlcNAcβ1-3) 1-3GalNAcβ1-4(GlcNAcβ1-3)0-1GlcNAcβ1- 6GalNAc-ol

Abstract:

Eggs from Amphibia are surrounded by several layers of jelly that are needed for proper fertilization. Jelly coat is composed of highly glycosylated mucin-type glycoproteins containing up to 60% of carbohydrates, which display a remarkable species-specificity. This material obtained from Triturus alpestris was submitted to reductive β -elimination and the released oligosaccharide-alditols were further fractionated by HPLC. Structural characterization was performed through a combination of two dimensional ¹H-¹H and ¹H-¹³C NMR and ESI-MS/MS analysis. Numerous carbohydrate chains are characterized by the presence of the Cad (Sd^a) determinant, including respectively NeuAc, NeuGc or Kdn as a sialic acid. But the most significant O-glycan sequences which mark the difference between the jelly of T. alpestris and other studies amphibian jellies are polymers of GalNAc(β 1-4)GlcNAc (LacdiNAc) which form part of the following sequence: HSO<inf>3</inf>(4)(GalNAcβ 1-4GlcNAcβ 1-3) <inf>1-3</inf>GalNAcβ 1-4(GlcNAcβ 1-3)<inf>0-1</inf>GlcNAcβ 1-6GalNAc-ol. © Springer Science + Business Media, LLC 2006.

Año de publicación:

2006

Keywords:

• ESI-MS/MS
• O-glycan
• Jelly coat
• NMR
• mucin
• Poly LacdiNAc
• Triturus alpestris
• Amphibia

Fuente:

scopus