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Surface Engineered Poly(lactide-co-glycolide) Nanoparticles for Intracellular Delivery: Uptake and CytotoxicityA Confocal Raman Microscopic Study

Abstract:

Confocal Raman Microscopy (CRM) is used to study the cell internalization of poly(lactide-co-glycolide) (PLGA) nanoparticles (NPs) fabricated by emulsion techniques with either poly(ethylene imine) (PEI) or bovine serum albumin (BSA) as surface stabilizers. HepG2 cells were exposed to PEI and BSA stabilized PLGA NPs. Spontaneous Confocal Raman Spectra taken in one and the same spot of exposed cells showed bands arising from the cellular environment as well as bands characteristic for PLGA, proving that the PLGA NPs have been internalized. It was found that PLGA NPs preferentially colocalize with lipid bodies. The results from Raman spectroscopy are compared with flow cytometry and confocal scanning laser microscopy (CLSM) data. The advantages of CRM as a label-free technique over flow cytometry and CLSM are discussed. Additionally, cell viability studies by means of quick cell counting …

Año de publicación:

2010

Keywords:

    Fuente:

    googlegoogle

    Tipo de documento:

    Other

    Estado:

    Acceso abierto

    Áreas de conocimiento:

    • Nanopartícula
    • Biotecnología

    Áreas temáticas:

    • Farmacología y terapéutica
    • Ingeniería y operaciones afines
    • Ingeniería química

    Contribuidores:

    Gabriela RomeroGabriela Romero