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Synthesis and luminescence properties of lanthanide(III) chelates with polyacid derivatives of thienyl-substituted terpyridine analogues

Abstract:

Two new polyacid derivative ligands of thienyl-substituted terpyridine analogues, N,N,N1,N 1-[4′-(2‴-thienyl)-2,2′:6′, 2″-terpyridine-6,6″-diyl]bis(methylenenitrilo) tetrakis(acetic acid) (TTTA) and N,N,N1,N 1-[2,6-bis(3′-aminomethyl-1′-pyrazolyl) -4-(2″-thienyl)pyridine] tetrakis(acetic acid) (BTTA), were synthesized, and the luminescence properties of their Eu3+ and Tb3+ chelates were investigated. The Eu3+chelates of the two ligands are strongly luminescent having luminescence quantum yields of 0.150 (TTTA-Eu 3+) and 0.114 (BTTA-Eu3+), and lifetimes of 1.284ms (TTTA-Eu3+) and 1.352ms (BTTA-Eu3+), whereas their Tb 3+ chelates are weakly luminescent. The TTTA-Eu3+ chelate was used for streptavidin (SA) labeling, and the labeled SA was used for time-resolved fluoroimmunoassay of insulin in human sera. The method gives the detection limits of 33pgml-1. © 2003 Elsevier B.V. All rights reserved.