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Synthetic seeds production and the induction of organogenesis in blackberry (Rubus glaucus Benth)

Abstract:

The present research consists in the production of artificial seeds and the induction of organogenesis in blackberry (Rubus glaucus Benth) starting from explants represented by apical and nodal segments. The disinfection process was achieved using different concentrations of sodium hypochlorite (0.5, 1 and 1.5%) with various immersion times (10 and 15 minutes), the best treatment being represented by the concentration of 1.5%. Shoots were induced from nodal and apical segments on MS basal medium containing various concentrations of TDZ (0.25, 0.5 0.75 and 1 mg. L-1), the treatment with 0.5 mg. L-1 TDZ having the best response. The explants were induced during the multiplication stage on MS basal medium containing various concentrations of AIA (0.5, 0.75 and 1 mg. L-1) associated with BAP (2 and 3 mg. L-1). The increased number of internodes and higher plants were achieved using the concentrations of 2 mg. L-1 BAP and 0.75 mg. L-1AIA. The optimal explants obtained were encapsulated in sodium alginate (3%) and calcium chloride (1%) dissolved on MS using different concentrations of brassinolide (0.1, 0.5 and 1 mg. L-1) and activated charcoal (0 and 1 g L-1) followed by the in vitro germination of blackberries using the treatment of BRA 1 mg. L-1 and 1 g L-1 activated charcoal.