T cell expansions in lymph nodes and peripheral blood in HIV-1-infected individuals: effect of antiretroviral therapy

Abstract:

Objective To evaluate dynamics in CD8 T cell expansions during highly active antiretroviral therapy (HAART). Design Various T cell subsets were isolated from blood and lymph nodes and analysed for T cell receptor (TCR) diversity. Methods TCR complementarity determining region 3 (CDR3) spectratyping and single-strand conformation polymorphism (SSCP) analyses were performed in combination with sequencing to assess clonality of the subsets. Results Strongly skewed CDR3 patterns in total CD8 cells and the CD8 subsets CD45RO+ CD27+ and CD45RO− CD27+ showed substantial dynamics in dominant CDR3 sizes, resulting in relative improvement of CDR3 size diversity in the first months of therapy. During sustained treatment, TCR diversity changed only moderately. SSCP profiles confirmed oligoclonality of TCR CDR3 perturbations. Various dominant CDR3 sizes for CD4 and CD8 T cells present in …

Año de publicación:

2001

Keywords:

Fuente:

google