Análisis químico y determinación de la actividad antioxidante de la fracción volátil destilada desde la especie orégano (Origanum vulgare) /
Abstract:
Abstract:The essential oil of the species Origanum vulgare, is isolated through steam distillation. The chemical composition of the essential oil was determined by gas chromatography coupled to GC/MS mass spectrometry and the CG/FID flame ionization detector. The biological activity was evaluated by microdilution method in broth and the antioxidant activity by two spectrophotometric methods: ABTS and DPPH. In addition, the physical properties of the essential oil were determined. Thirty compounds were identified that correspond to 98.58% of the total composition of the essential oil, the most representative compounds were: γ-Terpinene (22.75%), Linalool (21,05%), Terpinen-4-ol (9,50%), (Z) -β Ocimene (6.36%) and o-Cymene (6.06%). The oil density was 0.9250 g/cm3, the refractive index was 1.4792 and the optical activity was +14.4 °. The essential oil showed remarkable activity against Staphylococcus aureus ATCC 25923. Essential oil present antioxidant activity for both evaluated methods.
Año de publicación:
2018
Keywords:
- Ingeniero Químico.-
- Aceite esencial-
- Extracción de vegetales.-
Fuente:
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Tipo de documento:
Bachelor Thesis
Estado:
Acceso abierto
Áreas de conocimiento:
- Ciencia de los alimentos
- Bioquímica
- Bioquímica
Áreas temáticas:
- Química física
- Química orgánica
- Fisiología y materias afines