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The establishment of an In vitro culture protocol for Calamagrostis intermedia (J. Presl) steud. starting from meristems

Abstract:

The Andean Paramo is an important component of biodiversity, affected by the species inhabiting it and by the human activities. For these reasons the biotechnological technique represents an important tool for helping the conservation of this ecosystem, one of the alternatives being represented by the in vitro culture. This research aims to establish a protocol for in vitro micro propagation of Paramostraw (Calamagrostis intermedia) represented by the disinfection method and choose of the introduction, multiplication and rooting media, for short term conservative issues. For the disinfection phase were tested three concentrations of sodium hypochlorite(0.5%, 1%, 1.5%)(v/v) with different immersion time(10 to 15 minutes).The introduction media was represented by seven MS media with different concentration of vitamins: 0%, 15%, 30%, 45%, 60%, 85% and 100% (v/v). In multiplication and rootingphase was tested the influence of phytohormones such BAP (0.15 and 0.2 mg/L), BRA (2 and 3 mg/L) and KIN (0.05 and 0.1 mg/L). The best treatment for disinfection phase was represented by sodium hypochlorite 1% for 10 minutes. The important results for the micro propagation of this species consisted in using a concentration of vitamins of 30% (v/v) in MS medium during the introduction phase and 3 mg/L of BRA in the phase of multiplication and rooting.