Regresar

The inhibitory effect of 15-R-LXA <inf>4</inf> on experimental endometriosis

Abstract:

Objective: To determine the pro-resolution actions of 15-R-LXA 4 (LXA 4 ) on endometriotic lesions and on the expression of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in the murine endometriosis model. Study design: In a prospective, placebo-controlled experimental study, endometriosis was induced in thirty BALB/c mice, and fifteen sham-operated mice served as negative controls. Among the thirty mice with induced endometriosis, fifteen were administered 15-R-LXA 4 and acted as study group (LXA 4 group) and the other fifteen were used as positive controls (EM group). Then all the mice were sacrificed and the endometriotic lesions were weighed. The mRNA and protein levels of IL-1β and TNF-α in the peritoneal fluid were quantified by using real-time polymerase chain reaction (PCR) and enzyme-linked immunoabsorbent assay (ELISA). Result(s): Compared with the positive controls, 15-R-LXA 4 reduced the weight of the endometriotic lesions, decreased the concentrations of IL-1β and TNF-α, and lowered the mRNA levels of IL-1β and TNF-α in peritoneal fluid cells. Conclusion(s): These findings suggest that 15-R-LXA 4 inhibits the progression of endometriosis possibly by suppressing the gene and protein expression of IL-1β and TNF-α. © 2009 Elsevier Ireland Ltd. All rights reserved.