The role of glutathione-S-transferases in resveratrol transport out of grapevine cells

Abstract:

Vitis vinifera cell cultures response to elicitors synthesizing and extracellularly accumulating stilbenoid phytoalexins, particularly, large amounts of trans-resveratrol (tR) are produced when cells are challenged with methylated cyclodextrins (MBCD) alone or combined with methyl jasmonate (MeJA). The pathways for transport of tR to the extracellular medium in grapevine cells, being of utmost biotechnological relevance, are mostly unknown. Glutathione-S-transferases (GSTs) are a large protein family with different classes that carry out an array of functions including the trafficking and accumulation of plant secondary metabolites. Upon MBCD/MeJA treatments, both microarray transcriptome analysis (Almagro et al. 2014) and, especially DIGE proteome analysis (Martinez-Esteso et al. 2011; unpublished results), display strong abundance profile correlation of several t subclass GSTs with several stilbene synthase (StSy), and phenylalanine ammonia lyase (PAL) isoforms and paralogs and the tR metabolite itself. Further, qRT-PCR gene expression analysis provides evidence of co-expression of 5 StSy paralogs and 2 specific t-GSTs subclass paralog genes but not with 2 GSTs from other clades. This accumulated evidence strongly points to a role for GST in tR accumulation, potentially be related to the transport of the metabolite. To analyze the functionality of these specific GSTs, we have cloned one of the elicitor-induced grapevine GST genes and used for the generation of stably transformed grapevine cv Gamay cell lines using Agrobacterium-mediated transformation. In contrast to wild lines, only transformed cell lines were shown to accumulate …

Año de publicación:

2015

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Fuente:

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