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Total and soluble thiols in acid determination on the freshwater fish Colossoma macropomum (Cuvier, 1818) exposed to cadmium

Abstract:

In this study was evaluated the distribution of total thiols (TT) and acid soluble thiols (AST) in tissues of the freshwater fish Colossoma macropomum providing of cultivation and acclimated in laboratory by 15 and 30 days, as well, was evaluated the effect of cadmium on these parameters in Cd-exposure fishes during 21 days and in Cd-depuration fishes (15 days). TT and AST showed variations with environmental conditions; In Farmer fishes were, TT: plasm>muscle>gills; acclimated fishes (15 days): gills>muscle>liver>plasm>kidney and acclimated fishes (30 days): gills>kidney>liver>muscle>plasm. The distribution of AST was: farmer fishes: muscle>plasm>liver>gills. Acclimated fishes (15 days): liver>gills>muscle; the kidney and the plasm presented near values to zero, and acclimated fishes (30 days) was gills>muscle>liver>kidney>plasm. Cadmium exposition produced a significant increase in the TT and TSA concentration of plasm. Cd-depured fishes showed a decrease in plasm TSA concentration but not in plasm TT concentration. Thiol assays could be considered an important parameter in the diagnosis and monitor of fishes environmental Cd exposed.