Transfer and expression of marker (nptii) and reporter (gus) genes in atropa belladonna plants

Abstract:

An indirect, mediated genetic transformation of Atropa belladona vitroplants has been performed using the disarmed Agrobacterium tumefaciens pTi carrying the marker nptII gene for neomycinepliosphotransferase and the reporter gus gene for β-glucuronidase. Their expression into the plant host genome conferred the kanamycin resistance trait and respectively, the β-glucuronidase activity. These ones have been easily detected by both in vitro and molecular-genotype (PCR) and phenotype (protein)—assays. © 2003 Taylor and Francis Group, LLC.

Año de publicación:

2003

Keywords:

Fuente:

google

scopus