Trypanosoma vivax: A novel method for purification from experimentally infected sheep blood

Abstract:

Trypanosoma vivax is the principal etiological agent of bovine trypanosomosis, a widely disseminated disease in tropical and subtropical regions. Here, we present a simple and reproducible method for the purification of T. vivax from experimentally infected and immunosuppressed sheep, using an isopycnic Percoll gradient, followed by DEAE-cellulose chromatography, with an estimated yield of 11-15%. This method could be used for the purification of T. vivax geographical isolates from various locations and from different natural hosts. © 2005 Elsevier Inc. All rights reserved.

Año de publicación:

2005

Keywords:

• Trypanosoma vivax
• Immunosuppres-ovine
• purification
• Trypanosomosis

Fuente:

google

scopus