Use of a purified Trypanosoma cruzi antigen and CpG oligodeoxynucleotides for immunoprotection against a lethal challenge with trypomastigotes
Abstract:
The crucial role played by Ag163B6/cruzipain, the major cystein proteinase of Trypanosoma cruzi, in the process of parasite internalization into mammalian cells and IgG hydrolysis, signals this antigen as a potential target for raising a protective immune response against Chagas’ disease. On the other hand, synthetic oligodeoxynucleotides containing CpG-motifs (CpG-ODN) are capable of driving immunity toward a Th1 bias. Considering the importance of Th1 mechanisms in resistance against this intracellular parasite, we analyzed the ability of Ag163B6/cruzipain plus CpG-ODN to induce immunoprotection against a lethal challenge with trypomastigotes. Mice were immunized with Ag163B6+CpG-ODN showing high specific antibody titers, mostly IgG2a. Spleen cells from these mice strongly proliferated and presented significant increase of IL-2 and IFN-γ concentrations in their supernatant upon antigen …
Año de publicación:
2003
Keywords:
Fuente:

Tipo de documento:
Other
Estado:
Acceso abierto
Áreas de conocimiento:
- Inmunología
- Inmunología
- Microbiología
Áreas temáticas de Dewey:
- Microorganismos, hongos y algas

Objetivos de Desarrollo Sostenible:
- ODS 3: Salud y bienestar
- ODS 10: Reducción de las desigualdades
- ODS 17: Alianzas para lograr los objetivos
