Regresar

Use of urease type enzimes for the treatment of wastewater with a high organic nitrogen content

Abstract:

Some bacterial stock of the urease enzymes producer family were used, with the purpose of treating an industrial wastewater with urea content coming from a industrial urea complex, to reduce the organic nitrogen content to ammonia. To accomplish this objectives, a characterization of the wastewater was done, to identifies the organic nitrogen concentration present in the wastewater, the bacteria source generating the urease enzyme for the waste treatment were identified, and the conditions for the growth of the bacteria were established in the lab. The results show that it is possible the biodegradation using the urease enzymes kind, like Klebsiella Pneumoniae and Proteus Mirabilis, hydrolyzing the urea to ammonia and water. The Proteus Mirabilis was able to degrade the organic nitrogen with a conversion higher than 90%. From the kinetic study, the biodegradation reaction is a first order reaction with a constant kinetics rate of 0,4195 h-1 at 37°C.