Regresar

Validation and GMP production of mesenchymal stromal cells batches for clinical application in orthopedics and rheumatology

Abstract:

Results: Freshly harvested ASC displayed good viability up to 28 hours in albumin alone. Supplementation with glucose improved viability up to 48 hours. After 28 hours in storage medium, we noticed a decrease of proliferation and colony-forming unit fibroblasts potential associated with an increase of apoptosis. No significant changes in surface immunophenotype or differentiation potentials toward adipogenic, osteogenic and chondrogenic lineages were observed. Finally, liquid preservation resulted in a progressive decrease of immunosuppressive properties. Interestingly, 28-hours liquid-preserved cells recovered same properties as fresh cells after one passage in culture. On the other hand, cell cryopreservation induced a strong inhibition of immunosuppressive capacity of the ASC. Conclusion: We developed a GMP preservation protocol using human albumin supplemented with glucose allowing storage and …