Value of C3d assay and IgG subclass in the pbkp_rediction of the flow cytometry cross-match result for renal transplantation

Abstract:

The aim of this study is to compare the association and the pbkp_redictive capacity of DSA MFI, complement fixing capacity (C3d assay) and IgG subclasses determination in the pbkp_rediction of FCxM result. Methods We used cryopreserved (single bond70C) sera from potential renal transplant recipients, containing DSA against their respective potential donors. All patients showed negative AHG-CDC CxM and either positive or negative FCxM. Class I and Class II HLA-DSA were determined by Luminex SAB. C3d were detected by Luminex (Lifecodes®Immucor), DSA IgG 1–4 subclasses were evaluated using monoclonal antibodies specific for IgG subclasses (Luminex). Results 93 donor/recipient tests were evaluated; 32 (35.9%) patients presented at least one C3d + Ab, of which only 11 (11.8%) were donor specific. At least one IgG subclass was identified in 45 samples (48.3%). Twenty-seven FCxM tests (29%) were …

Año de publicación:

2018

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Fuente:

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