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Variability in expression profiles of Betulaceae spring pollen allergens in Central Europe region

Abstract:

Pollen allergies are an important and relevant question in the personal life of millions of people. They are well characterized on the genomic and proteomic level of allergens per se and on medicinal level, but the very few information exists about the natural variability and background of pollen allergen transcription patterns. Here, the variability of expression of Bet v 1, Bet v 2, Cor a 1 and Cor a 2 were performed. Both of the analysed species, Betulla verrucosa as well as Corylus avellana are clinically relevant in spring pollinosis in the area of central Europe. Bet v 1 and Bet v 2 expressions were subjected to compare in pollen for a total of twelve accessions from different areas. Cor a 1 and Cor a 2 expressions were subjected to comparison in pollen for a total of thirty-one natural pollen germplasm accessions. Expression changes of Bet v 1 were changed in three different manners – in six pollen samples was the expression higher than 1.5, three pollen samples have no relevant change and in three pollen samples was the expression lower about 0.5 times. Expression changes of Bet v 2 were as follows - five of the analysed samples were expressed more than 3 times when compared to the calibrator and in all of the other samples was the expression change in the difference that was not above 0.7 times. Expression changes of Cor a 1 were very similar among themselves and only in five samples was expression relevantly higher with at least 1.1 fold change. Expression changes of Cor a 2 were very similar among themselves and only in four samples was expression relevantly higher with at least 1.4 fold change.