Variante de la tinción de Nitrato de Plata Amoniacal para destacar placas densas (Fibronexus) del Miofibroblasto

Abstract:

Introduction: the use of silver stainings were developed in the XIX century, at the beginning were used to stain nervous tissues, and had a major role in the postulate of cellular theory as a biological dogma. After, It was used in connective tissue, but it have never been used in identification of myofibroblasts. Connective tissue cells with many functions and related with fibrosis. Objective: To determine the usefulness of a variant of the ammoniacal silver nitrate technique in selective staining of the fibronexus, an ultrastructural characteristic of myofibroblasts, in the renal capsule of Wistar rats. Materials and methods: Immunohistochemistry and electron microscopy were used to determine the presence of myofibroblasts in the renal capsule of Wistar rats. Finally, the variant of the ammoniacal silver nitrate technique. Results: the argyrophil plaques were found in myofibroblasts of the renal capsule which correspond to those ones in immunohistochemistry and electron microcscopy. Conclusions: the variant of the ammoniacal silver nitrate technique is useful in the selective staining of subplasmalemmal dense plaques in myofibroblastic morphology. However, it is still necessary to clarify its basis and the testing of the technique in other tissues. Resumen Introducción:: El uso de tinciones con sales de plata fue desarrollado en el siglo XIX, al principio fueron usadas para teñir tejido nervioso y tuvo un papel preponderante en el postulado de la teoría celular como dogma biológico. Posteriormente, se desarrolló su uso en tejido conectivo, pero nunca fueron utilizadas en la identificación de miofibroblastos, células del tejido conectivo con funciones muy diversas …

Año de publicación:

2017

Keywords:

Fuente:

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