Applying SWATH mass spectrometry to investigate human cervicovaginal fluid during the menstrual cycle

Abstract:

Inherent interindividual and intraindividual variation in the length of the menstrual cycle limits the accuracy of pbkp_redicting days of peak fertility. To improve detection of days of peak fertility, a more detailed understanding of longitudinal changes in cervicovaginal fluid (CVF) biomarkers during the normal menstrual cycle is needed. The aim of this study, therefore, was to characterize longitudinal changes in CVF proteins during the menstrual cycle using a quantitative, data-independent acquisition mass spectrometry approach. Six serial samples were collected from women (n = 10) during the menstrual cycle. Samples were obtained at two time points for each phase of the cycle: early and late preovulatory, ovulatory, and postovulatory. Information-dependent acquisition (IDA) of mass spectra from all individual CVF samples was initially performed and identified 278 total proteins. Samples were then pooled by time …

Año de publicación:

2015

Keywords:

Fuente:

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