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Association of the HLA‐G 14‐bp insertion/deletion polymorphism with juvenile idiopathic arthritis and rheumatoid arthritis

Abstract:

We tested the possible association of the 14‐bp polymorphism of the HLA‐G gene in the course of two inflammatory diseases, rheumatoid arthritis (RA) and juvenile idiopathic arthritis (JIA). Patients and controls were genotyped for the 14‐bp polymorphism by polymerase chain reaction with specific primers for the exon 8 of the human leukocyte antigen (HLA)‐G gene and the amplified fragment was visualized in a 6% polyacrylamide gel. A total of 106 JIA patients, 265 RA patients, 356 healthy adults and 85 healthy children were genotyped for the 14‐bp polymorphism. Female JIA patients presented a higher frequency of the −14 bp allele when compared with female healthy children (0.743 and 0.500, corrected P = 0.003), which reflected in the JIA group as a whole. This increased frequency of the −14‐bp allele was observed in all JIA subtypes. In RA patients, no differences in allelic and genotypic frequencies …