Bacteriophages: From phage therapy to combinatorial biology

Abstract:

Bacteriophages (phages) are the most studied viruses and the major contributors for the development of Molecular Biology. Their lytic action over pathogenic bacteria makes them useful as an alternative therapy for the treatment of bacterial infections. Their efficacy has been proven even against several drug-resistant bacteria. Phage display of a wide range of polypeptides has been increasingly used to identify novel molecules with useful binding properties for research, medical and industrial applications. Bacteriophage researchs have improved the sophistication of methods used for phage display of proteins. These biological libraries will probably give a solution for most of the binding problems between molecules, and the best application would be that exploring this difficulty by means of combination of selection rounds (Biopanning) and determination of DNA sequences. This technology has the potential to generate vast databases containing relationships between protein structure and biological activity.

Año de publicación:

2001

Keywords:

• Combinatorial libraries
• phage display
• Phage therapy

Fuente:

scopus